RR99821 - Phase Separation 6ml, pk/100

Phase Separation Columns
The Phase Separation Columns contain a hydrophobic frit and are designed to separate organic phases that are denser than water (eg dichloromethane).

They can be used in the ‘Lower Level’ if further purification is not required or in the ‘Upper Level’ if SPE purification is required after phase separation.

Following partition of a crude reaction mixture between for example dichloromethane and water, the mixture is transferred to a Phase Separation Column where the lower organic layer will run through the frit leaving the upper aqueous layer for disposal. It should not be necessary to apply pressure, indeed doing so can allow the aqueous layer to break through the hydrophobic frit.